The extract from carrot was more heatstable than other extracts. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. Antibacterial activity of crude extracts of selected plants. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. In vitro antifungal activity screening of crude extracts of soil fungi against plant pathogenic fungi n.
Trolox tr was used as a standard, and the totalantioxidant capacity of plant extracts were measured as mm tr equivalent. Antimicrobial activity of wild olive crude extracts in vitro. Studies on some plant extracts for their antimicrobial. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Antifungal activity of plant extracts against dermatophytes article pdf available in mycoses 421112. Antioxidant activity was studied using dpph, cuprac, reducing power assay, deoxyribose degradation site and nonsite specific, ferric reducing antioxidant potential. Antibacterial activities of plant extracts were compared with those of standard antibiotics. Antifungal activity of medicinal plant extracts against phytopathogenic fungus alternaria spp. Antibacterial, antioxidant, and anticholinesterase activities of plant. Assessment of antioxidant activity of plant extracts by. Antifungal activity of some plant extracts against guava.
Evaluation of nematicidal activity of ethanol extracts of. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. Antioxidant, antihypertensive, antihyperglycemic, and. These results show that the aqueous extracts of five native plants of. Fresh leaves of eleven different plant species free from. Antifungal activity of some medicinal plant extracts. Kijjoab, c abstract the ethyl acetate extracts of the culture of eupenicillium parvum, gelasinospora. Antibacterial and antifungal activity of plant extracts 229 glaucus, aspergillus niger, botritis cinerea, cladosporium herbarum, fusarium culmorum, fusarium oxysporum, fusarium poae, fusarium. Screening of antibacterial activity of the medicinal plant.
These data indicate that selected plant extracts are potential sources of dietary antioxidants. All plant hydroalcoholic extracts contained the expected phytochemical markers for each species, confirming their botanical identity data not shown. Antifungal activity of plant extracts with potential to. Plant extracts with biofilm inhibitory activity biofilms are the default modeoflife for many bacterial species and biofilmbased infections. The extracts that presented antimicrobial activity were tested for hemolytic assay against human a, b, and o blood types and rabbit blood. In order to extract different phenolic compounds from plants with a high degree. The phenol and flavonoid contents of the plant extracts were in the range 20. The antibacterial activity of plant extracts of the lamiaceae family was evaluated against clinical isolates of multiresistant gramnegative bacteria by broth. Cold and hot water leaf extracts of 25 plant species comprising horticultural crops including multipurpose trees, weeds, forage crops and medicinal plants at 5% group i and 30 forest tree species at 10% group ii were tested against the brinjal aubergine, solanum melongena damping off pathogen, pythium aphanidermatum in pda cultures. Antifungal activity of some plant extracts against. Comparisons with pertinent data from literature indicate that, according to the methodology adopted in studies on antimicrobial activity, the most diverse results can be obtained.
Effect of combined active plant parts extracts on antimicrobial activity combinations of promising extracts were assessed as previously described by berenbaum17. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. In the present study, methanol and aqueous extracts of parkinsonia aculeata l. Antifungal activity of some plant extracts on some pathogenic fungi article pdf available in archives of phytopathology and plant protection 473 february 2014 with 1,206 reads. Several assays have been frequently used to estimate antioxidant capacities in. Antimycotic activity of the extract against the three dermatophytes varied significantly p test plants. Phytochemicals, antioxidant and antimicrobial potentials. Phytochemical investigation and biological activity of. Consequently, the search and study of plant extracts that have the highest antiradical and antioxidant activity aoa is a very urgent task. Effect of combined extracts from different plant parts of.
As well, the smallest concentration capable of inhibiting or preventing growth was determined among the species and extracts that demonstrated inhibitory properties. Antibacterial activity of lamiaceae plant extracts in clinical. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Pdf antifungal activity of plant extracts with potential. The development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal. Assessment of antioxidant activity of plant extracts. A number of studies have investigated the activity of plant extracts and products against specific oral pathogens, while others have focused on the ability of the products to inhibit the formation of dental biofilms by reducing the adhesion of microbial pathogens to the tooth surface, which is a primary event in the formation of dental plaque.
Four different solvents were used for the extraction. The antifungal activity of the methanol extracts of six medicinal plants used in native medicine in sri lanka is reported. The antibacterial activity of extracts of nine plant species with good. Punjaisee, department of clinical microbiology, faculty of associated medical sciences, chiang mai university c.
Comparative study of antioxidant properties and total. Screening of in vitro antioxidant activity of methanolic. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. An increased inhibitory effect of the mycelial growth was observed with the increase in concentration from 10 to 50%. Antibacterial and antifungal activities from leaf extracts. Indeed, for use in food and nutraceuticals, aqueous plant extracts are nutritionally more relevant and would have obvious advantages in relation to certi. Determination of the mics of hydroalcoholic extracts of the 14 canadian botanicals against ng isolates revealed a range of inhibitory activities. Antifungal activity of plant extracts with potential to control plant pathogens in pineapple article pdf available in asian pacific journal of tropical biomedicine 61 november 2015 with. By ngaruiya paul njuguna a thesis submitted in partial fulfilment of master of. Antimicrobial activity of single and combined extracts of. For measuring radical scavenging activity rsa against the stable radical n,n diphenylnpicrylhydrazyl dpph, 0.
None of the aqueous extracts showed antimicrobial activity. The effect of various plant extracts was measured on several important oxidation indicators. Many plant extracts and essential oils have been screened for their nematicidal activity against the plantparasitic nematodes814. The analysis of bioactive compounds present in the plant extracts involving. Antioxidant activity of herbaceous plant extracts protect. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated as abts radical scavenging activity % abs control. Numerous works devoted to antioxidant activity of plant extracts are published. Ten dif ferent plants were collected from in and around sivakasi area.
In these works aoa are given in units % mg, mgml or mgg, that character. The chemical examination follows after the isolation and biological screening of the active fraction1. Antifungal activity of plant extracts with potential to control plant pathogens in pineapple. An invitro study abstract plant derived products have been used for medicinal purposes for centuries. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. Antifungal activity of wild sage lantana camara against colletotrichum falcatum a. Enzyme inhibitory properties, antioxidant activities, and.
Evaluation of antioxidant activity of some plant extracts. Methanol, ethylacetate extracts of plant species heracleum crenatifolium, h. Phytochemical screening of plant extracts especially those which have been used in traditional medicine is therefore essential so as to identify phytochemical constituents in the plants that are responsible for a given bioactivity. Verma 1, pradeep babu 2, vinay gupta 2, gbks prasad 3 and v. Extracts of canadian first nations medicinal plants, used. Bioactive aqueous extracts of twelve yucatecan plants. The components showed slight antimicrobial activity ahmed et al. The antifungal activity of extracts from datura stramonium, lawsonia alba, mentha piperita, eucalyptus citriodora, kigelia pinnata, lantana camara, azadirachta indica, canna indica, allium cepa, portulaca indica, ziziphus jujuba, prosopis spicigera, calotropis procera and other plants against alternaria brassicae, colletotrichum papayae and helminthosporium sp. Antimicrobial activity, toxicity and phytochemical. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Antibacterial characheristics of plants from heracleum genus have relatively slightly been researched. All plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution mid against above fungi. In traditional indian medicine or ayurveda, zingiber officinale and many other herbs have been used as medicine. Plant extracts 1 ml were allowed to react with 1 ml of the abts solution and the absorbance was taken at 734 nm after 7 min using the spectrophotometer.
Each purified extracts were dissolved in dimethyl sulfoxide, sterilized by filtration using sintered glass filter, and stored at 4c. Plant extracts have shown inhibitory effect on the growth of. Extraction, isolation and characterization of bioactive compounds. Antibacterial activities of some borneo plant extracts. Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita. Euphorbia fischeriana steud is a perennial herbaceous plant with latex, distributed mainly in north china 15. The capacity of the extracts and antibiotics were evaluated on the.
In vitro antifungal activity screening of crude extracts. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Khamwan microbiology section of the central laboratory service unit faculty of medicine. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens. Evidencebased complementary and alternative medicine. Briefly, a twodimensional, twoagent broth microdilution checkerboard technique was used to study the interaction between the promising extracts. Review cytotoxic studies of plant extracts cytotoxicity of plant extracts on both plant and animal test material is capturing scientific interest, as it can indicate their antitumor and anticarcinogenic potentials, which have always been a gateway for the development of novel anticancer drugs. All the extracts were assayed for their antibacterial activities in a standard minimum inhibitory concentration mic assay.
Antioxidant activity of methanol extracts of different. Efficacy of plant extracts in plant disease management. Antifungal activity of some plant extracts against clinical pathogens abhishek mathur 1, ravinder singh 1, sajad yousuf 1, aprajita bhardwaj 1, satish k. Results of antimicrobial activity of the five plant extracts can suggested that s. In the present study, we evaluated the potential of 5 selected plants to inhibit test bacteria by agar well diffusion assay and the result is shown in table 2.
The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Extracts of capparis spinosa and juglans regia completely prevented growth of m. Antioxidant properties of the methanol extracts of the. Phytochemical analysis of phyllanthus amarus extracts s.
The methanolic extract of caryophyllus aromaticus presented the highest antis. Antibacterial activity of medicinal plant extracts. All plant extracts used had shown antifungal activity against fusarium solani. Antifungal activity of plants extracts were determined by agar plate methods. Evaluation of some plant extracts for their nematicidal.
Different dose of plant extracts 50, 100, 200 and 400 mgml were applied against these plant pathogens and the value of mycelial growth inhibition mgi and mycelial growth mg were recorded. Pdf antibacterial and antifungal activity of plant extracts. Antifungal activity of some medicinal plant extracts against candida albicans and cryptococcus neoformans s. Antibacterial and antifungal activity of plant extracts 221 synthesis of the obtained results t able 6 showed that the activi ty of the extracts depended. Pdf antifungal activity of some plant extracts on some. Antifungal activity of ginger extract on candida albicans. Antimicrobial activity of some plant extracts against. Antifungal activity of wild sage lantana camara against. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. A comparative evaluation of antifungal activity of. Antioxidant activity of plant extracts containing phenolic compounds.
Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of punica granatum, known as pomegranate, had their antifungal activity tested against candida spp. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. This is a good study in which the authors evaluated the antioxidant activity of extracts of root, stem, leaf, flower and fruit of l.
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